Antimicrobial activity of Plumbago zeylanica plant extracts and its application in water and laboratory disinfection

Abstract

This study was carried out to investigate the antimicrobial activities/ potentials of Plumbago zeylanica components (leaf, stem and root) on four bacterial species, Baccilus subtilis, Staphylococcus aureus, Escherichia coli and Klebsiella pneumonia with the aim of using the active part of the plant to be used in water and laboratory disinfection. The plant parts crude extract was concentrated using a rotary evaporator and dried in a freeze drier. Different concentrations of the plant parts were then prepared from the dried plant extract and tested on the four pathogens using agar diffusion methods. The results indicated that active antimicrobial properties are concentrated more in the roots been very effective against Escherichia coli even at low concentration. However, at higher concentration all the plant extracts became effective against the bacteria. The study concludes that the roots of Plumbago zeylanica possess the highest antimicrobial potentials for disinfection. Then, the root extract was tested on effluent water and the results showed significance reduction level of Escherichia coli.